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Download Advances in lipid research. Vol. 18, 1981 by Rodolfo Paoletti; David Kritchevsky PDF

By Rodolfo Paoletti; David Kritchevsky

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It has also helped in identifying the cells involved in the early proliferative lesion (Thomas et al, 1971). Autoradiographic techniques can also be used in conjunction with electron microscopy (Caro et al, 1962; T h o m a s et al, 1968). D. IMMUNOCHEMICAL T E C H N I Q U E S Microscopic examination of the atherosclerotic lesion reveals the presence of a variety of c o m p o n e n t s , such as lipid, calcium, fibrin, and ground substance. ). However, other plasma proteins k n o w n t o penetrate the endothelium cannot be identified by conventional histological methods.

Electron microscopic sen­ sitivity of the concanavalin A method is increased by using the fact that it has t w o sites reactive t o glucoproteins. Thus it can be fixed t o sugar at one of these active sites, while the other active site remains free. This free site acts as a receptor for secondary sugar in the system, such as horseradish peroxidase. T h e catalytic activity of the peroxidase molecule is then re­ vealed by the diaminobenzidine (DAB) method of G r a h a m and Karnovsky (1966). A n overview of the characteristics of concanavalin A was published recently by C h o w d h u r y and Weiss (1975).

This tech­ nique offers an additional dimension of visual interpretation of lesions. However, it has complicated the interpretation a n d analysis of this dynamic process even m o r e due t o an even greater sampling limitation. 32 DRAGOSLAVA VESSELINOVITCH AND KATTI F I S C H E R - D Z O G A A n excellent summary of the present day knowledge about ultrastructure in general and the circulatory system in particular (including methodology) can be found in Constantinides' " F u n c t i o n a l and Electronic H i s t o l o g y " (1974).

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